OBJECTIVE: The aim of the present study was to determine the prevalence of sero positivity for HBsAg, Anti-HBcAg and AntiHVC in the blood bank of Hospital Daniel Carrion during the period 2010 - 2012. MATERIALS AND METHODS: Retrospective cross-sectional study. Potential donors who met the inclusion criteria were included. Sociodemographic factors, risk behaviors were gathered. A descriptive analysis was performed with STATA 14. RESULTS: 13,887 potential blood donors of the HNDAC between January 2010 and December 2012 were identified. The population's mean was 37 years, 32% were women. 897 potential positive blood donors were identified. The prevalence of HBsAg was 0.55%; Anti-HBcAg, 5.15%; and Anti-HVC, 1.25%. CONCLUSION: The prevalence of positive serology for HBsAg was similar to the previous reports and Anti-HVC was higher than the prevalence reported in our country.