Title
In vitro characterization of indigenous probiotic strains isolated from colombian creole pigs
Date Issued
01 July 2020
Access level
open access
Resource Type
journal article
Author(s)
Zamorano University
Publisher(s)
MDPI AG
Abstract
Three lactic acid strains were isolated from feces of the native Zungo Pelado breed of pigs (n = 5) and presumably identified as belonging to the Lactobacillaceae family by morphological techniques showing that they were Gram‐positive/rod‐shaped and catalase‐ and oxidase‐negative. They were then identified by biochemical tests using API 50CHL as Lactobacillus plantarum (CAM6), Lactobacillus brevis (CAM7), and Lactobacillus acidophilus (CL4). However, 16S rRNA identification showed that all three strains were Lactobacillus plantarum. Additionally, all three isolates were able to grow in pH 3 and 4. Interestingly, the growth of the CAM7 strain decreased at pH 5.6 compared to that of the CAM6 strain (p < 0.05), and the growth of the CL4 strain was reduced at pH 7(p < 0.05). All three candidates showed good growth on bile salts (≥0.15%), and CAM6 and CAM7 showed better tolerance at higher concentrations (0.30%). Similarly, all strains tolerated sodium chloride (NaCl) concentrations from 2 to 10%. These strains also grew well at all temperatures tested (30, 37, and 42 °C). The CAM6 strain showed in vitro antibacterial activity against selected enteropathogenic bacteria (Escherichia coli strain NBRC 102203 and Salmonella enterica serovar Typhimurium 4.5.12) and commensal bacteria (Klebsiella pneumoniae ATCC BAA‐1705D‐5 and Pseudomonas aeruginosa ATCC 15442) and resistance to all antibiotics except amoxicillin. Further studies to evaluate the effects of these probiotic candidate strains in commercial pigs are currently underway.
Start page
1
End page
11
Volume
10
Issue
7
Language
English
OCDE Knowledge area
Biotecnología agrícola
Biología celular, Microbiología
Ciencia veterinaria
Scopus EID
2-s2.0-85088407117
Source
Animals
ISSN of the container
20762615
DOI of the container
10.3390/ani10071204
Source funding
Universidad de Córdoba
Sponsor(s)
Acknowledgments: This study was supported by University of Córdoba, Colombia, Grant No. FINV‐011. The authors would like to extend their sincere appreciation to the specialists and technicians and very special acknowledgement to the late Cecilia Lara of the GRUBIODEQ group, Faculty of Sciences, University of Córdoba, Colombia.
Funding: This work was supported by the University of Cordoba, Colombia under grant agreement number FCB‐06‐16.
Sources of information:
Directorio de Producción Científica
Scopus