Serra Negra is a region surrounded by some stretches of mountain, covered by Atlantic Forests and cloud scrubs, located in the southern part of Zona da Mata of Minas Gerais, in Serra da Mantiqueira, between the rises of the massif of Itatiaia (RJ, SP e MG) and the Serra do Ibitipoca (MG). The aim of this study was to determine the composition, structure and floristic similarity of arboreal flora among three forest types of Atlantic Forest (Alluvial, Montane and Cloud Forest) and also the similarity with other studies. A total of 2,572 individuals was sampled, from 194 species, 59 families and 118 genera. The highest number of species was recorded in the families Myrtaceae (30 morphospecies), Lauraceae (20), Melastomataceae (17) and Fabaceae (13). The three vegetation types associated with different environmental conditions differ in their composition and structure. The alluvial proved with low diversity and stature and high ecological dominance. The cloud forest stood out for its typical elements of altitude over other common species in the area below the range, where diversity was higher. The tree flora of Sierra Negra presents various indicator species to characteristic distribution of highlands of southeastern Brazil.