The objective of this study was to evaluate the dry matter intake (DMI) and neutral detergent fibre intake (NDFI) in the alpaca according to various levels of neutral detergent fibre (NDF) in the diet, and to estimate the intake based on NDF content in the diet. Four 3-year-old male Huacaya alpacas of 48.6 ± 1.3 kg body weight (BW) were used in a 4x4 Latin Square design. Feed was supplied ad libitum twice daily at 08:00 and 16:00 h. The treatments, based on oat hay with different proportions of stems and leaves, processed by a grinder at the size of 2 cm and separated by a sieve of 0.4 cm, were: leaves of oat hay (T1), 50% of oat hay and 50% of leaves of oat hay (T2), whole oat hay (T3) and stems of oat hay (T4), corresponding to 58.24, 61.38, 66.31 and 70.22% of NDF respectively. The results showed that increasing the level of NDF in the diet causes a decrease on DMI (p<0.05) and without affecting NDFI. The maximum NDFI was 0.97% BW and 25.6 g/kg0.75. The average NDFI was 0.92 ± 0.06% BW and 24.22 ± 1.62 g/kg0.75. In conclusion, NDF in the range used in the study affects feed intake in the alpaca.