A radar equipment was used to measure the deflection response of bridge stay-cables induced by ambient and traffic excitation. After a concise description of the radar equipment and a summary of advantages and potential issues of the microwave technology, the paper focuses on the experimental tests performed on all stay-cables of the curved cable-stayed bridge erected in the commercial harbor of Porto Marghera, Venice, Italy. The bridge consists of an inclined concrete tower, single-plane cables and a composite deck; the curved deck has a centerline length of 231 m, with two different side spans and 9 cables supporting each side span. Three series of ambient vibration tests were performed (on July 2010, April 2011 and October 2019) on the two arrays of cables of the bridge by using conventional accelerometers and microwave interferometer. The availability of simultaneously collected radar and accelerometer data (which are usually regarded as reference data in dynamic tests) allowed to investigate the accuracy of the radar technique (in terms of natural frequencies and tensile force estimated from natural frequencies) and the errors/uncertainties in radar results. Furthermore, the tests allowed to verify the repeatability of radar survey, with SHM purposes.