Title
Molecular and antigenic characterization of group C orthobunyaviruses isolated in Peru
Date Issued
01 July 2018
Access level
open access
Resource Type
journal article
Author(s)
Caceda R.
Huaman A.
Williams M.
Hang J.
Juarez D.
Kochel T.
Halsey E.
Forshey B.
Unidad de Investigación Médica Naval-6
Publisher(s)
Public Library of Science
Abstract
Group C orthobunyaviruses (GRCVs) are a complex of viruses in the genus Orthobunyavirus and are associated with human febrile disease in tropical and subtropical areas of South and Central America. While numerous GRCVs have been isolated from mosquitoes, animals, and humans, genetic analysis of these viruses is limited. In this study, we characterized 65 GRCV isolates from febrile patients identified through clinic-based surveillance in the northern and southern Peruvian Amazon. A 500 base pair region of the S segment and 750 base pair regions of the M and L segments were sequenced. Pairwise sequence analysis of the clinical isolates showed nucleotide identities ranging from 68% to 100% and deduced amino acid sequence identities ranging from 72% to 100%. Sequences were compared with reference strains of the following GRCVs: Caraparu virus (CARV), Murutucu virus (MURV), Oriboca virus (ORIV), Marituba virus (MTBV), Itaqui virus (ITQV), Apeu virus (APEUV), and Madrid virus (MADV). Sequence comparison of clinical isolates with the prototype strains based on the S and L segments identified two clades; clade I included isolates with high genetic association with CARV-MADV, and clade II included isolates with high genetic association with MURV, ORIV, APEUV, and MTBV. Genetic relationships based on the M segment were at time inconsistent with those based on the S and L segments. However, clade groupings based on the M segment were highly consistent with relationships based on microneutralization assays. These results advance our understanding of the genetic and serologic relationships of GRCVs circulating in the Peruvian Amazon.
Volume
13
Issue
7
Language
English
OCDE Knowledge area
Física atómica, molecular y química
Bioquímica, Biología molecular
Scopus EID
2-s2.0-85050515942
PubMed ID
Source
PLoS ONE
ISSN of the container
19326203
Sponsor(s)
The study was supported by the United States Department of Defense Global Emerging Infections Surveillance and Response System, a Division of the Armed Forces Health Surveillance Center Work Unit Number: 847705.82000.25GB. B0016. We would like to thank Christopher Mores, Marita Silva, Carolina Guevara and Dina Popuche from NAMRU-6, also Pablo Ramirez from San Marcos University for invaluable support. Disclaimers: The views expressed in this article are those of the author and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government. Copyright statement: Some authors of this manuscript are military service members or employees of the U.S. Government. This work was prepared as part of their official duties. Title 17 U.S.C. § 105 provides that ‘Copyright protection under this title is not available for any work of the United States Government’. Title 17 U.S.C. § 101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person’s official duties.
Sources of information:
Directorio de Producción Científica
Scopus