Nine wild Bornean leopard cats were anesthetized using a combination of tiletamine and zolazepam (Zoletil©) after being captured in humanely-designed live traps in Sabah, Malaysian Borneo, for the purpose of fitting radio-collars. For five leopard cats (group 1) a single dose of 6.92±1.06 mg/kg of Zoletil© was administered. The mean induction time from the initial Zoletil© dose was 7.9 ± 1.77 minutes, and the mean anesthesia time was 47.2 ± 25.1. For 4 leopard cats (group 2) after an initial mean dose of 6.92±1.06 mg/kg of Zoletil©, it was necessary to administer a second dose (or booster) of Zoletil© (mean dose 2.6±0.33 mg/kg) or ketamine (mean dose 3.5± 0.05mg / kg) to achieve complete immobilization. There were differences between the periods of anesthesia resulting from these boosters, which were 43.5 ± 2.1 minutes for ketamine and 89.5 ± 6.36 minutes for Zoletil©. We conclude that an initial dose of Zoletil© of 6.92 mg/kg can produce an adequate plane of anaesthesia without needing additional or booster injections of anaesthetic; if a booster is required, the use of ketamine in preference to Zoletil© has the benefit of shorter release times (245 minutes for ketamine booster compared to 350 minutes for Zoletil© booster) whilst providing adequate anesthetic times (mean 43.5 minutes for ketamine booster).