This work describes the construction and application of a biomimetic sensor for paracetamol determination in different samples . The sensor was prepared by modifying a glassy carbon electrode surface with a Nafion® membrane doped with FeTPyPz. The best performance of the sensor in 0.1 mol L-1 acetate buffer was at pH 3.6. Under these conditions, an oxidation potential of paracetamol was observed at 445 mV vs. Ag|AgCl. The sensor presented a linear response range between 4.0 and 420 μmol L-1, a sensitivity of 46.015 mA L mol-1 cm-2, quantification and detection limits of 4.0 μ mol L-1 and 1.2 μmol L-1, respectively. A detailed investigation about its electrochemical behavior and selectivity was carried out. The results suggested that FeTPyPz presents catalytic properties similar to P450 enzyme for paracetamol oxidation. Finally, the sensor was applied for paracetamol determination in commercial drugs and for the monitoring of its degradation in an electrochemical batch reactor effluent. ©2008 Sociedade Brasileira de Química.