Title
Detection of Mycobacterium tuberculosis DNA in buccal swab samples from children in Lima, Peru
Date Issued
01 January 2020
Access level
open access
Resource Type
journal article
Author(s)
Mesman A.W.
Soto M.
Coit J.
Aliaga J.
Mendoza M.
Leon S.R.
Mestanza F.M.
Mendoza C.J.
Murray M.B.
Holmberg R.C.
Pollock N.R.
Franke M.F.
Socios en Salud Sucursal Peru
Socios en Salud Sucursal Peru
Publisher(s)
Lippincott Williams and Wilkins
Abstract
We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).
Start page
E376
End page
E380
Language
English
OCDE Knowledge area
Enfermedades infecciosas
Sistema respiratorio
Pediatría
Subjects
Scopus EID
2-s2.0-85093908062
PubMed ID
Source
Pediatric Infectious Disease Journal
ISSN of the container
08913668
Sponsor(s)
The authors declare no conflicts of interest. This work was supported by the National Institutes of Health under the Center of Excellence in Translational Research (CETR) grant U19 AI109755. J.A.F. is a doctoral student studying Epidemiological Research at Universidad Peruana Cayetano Heredia under FONDECYT/Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica (FONDECYT) scholarship EF033-235-2015. The other authors have no funding or conflicts of interest to disclose.
Sources of information:
Directorio de Producción Científica
Scopus