The goal of this work was to study the effect of bacteria adaptation - To the presence of a mineral substrate - in apatite and quartz bioflotation. Zeta potential studies, surface tension measurements and microflotation were used to evaluate the performance of the microorganism. The electrophoretic studies showed a little change in the isoelectric point (IEP) value of the bacteria after the adaptation. Before the cellular adaptation, it was observed a flotability of the apatite around 45% at pH 5, with 0.15 g/L of bacteria after 2 min. of flotation. While, quartz achieved only 14%. Moreover, when the mineral-adapted cells were used, an improvement in mineral flotability values was observed. The apatite flotability increased when apatite-adapted R. opacus was used, furthermore the same behavior was observed in the case of quartz flotability. This effect was more pronounced in acidic pH range.