Title
Development and evaluation of porcine cysticercosis quickelisa <sup>™</sup> in triturus<sup>®</sup> EIA analyzer
Date Issued
01 January 2010
Access level
metadata only access
Resource Type
journal article
Author(s)
Handali S.
Pattabhi S.
Lee Y.M.
Silva-Ibanez M.
Kovalenko V.A.
Levin A.E.
Roberts J.M.
Hancock K.
Tsang V.C.W.
Centers for Disease Control and Prevention
Johns Hopkins University
Abstract
We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.
Start page
60
End page
70
Volume
31
Issue
1
Language
English
OCDE Knowledge area
Parasitología
Subjects
Scopus EID
2-s2.0-77951244786
PubMed ID
Source
Journal of Immunoassay and Immunochemistry
ISSN of the container
15321819
Sources of information:
Directorio de Producción Científica
Scopus