Title
Effects of two semen extenders on motility and integrity of sperm membrane in ovine frozen semen
Other title
Efecto de dos dilutores sobre la motilidad e integridad de la membrana espermática en semen congelado de ovinos
Date Issued
01 January 2010
Access level
metadata only access
Resource Type
journal article
Publisher(s)
Universidad Nacional Mayor de San Marcos
Abstract
The objective of the study was to evaluate the effects of two semen extenders: Tris-Fructose-egg yolk (Tris) and Citrate-Glucose-egg yolk (citrate) on motility and sperm membrane integrity (HOST) in ovine frozen semen in pellets. The study was carried out at the Semen Bank of the Agrarian University La Molina, in Lima, Peru, using 4 rams (2 Assaf and 2 Blackbelly) of 3.5 to 4 years old. A covariance analysis was used to evaluate the effect of the treatment and breed on Individual Progressive Motility (IPM), and randomized block design to evaluate the effect of extenders on sperm membrane integrity. Semen was frozen of dry ice and thawing was done in test tubes at 38 °C. In the Assaf breed, IPM of thawed semen was 63.77 and 61.11% when using Tris and citrate respectively, showing statistical difference (p<0.05). In the Blackbelly breed the IPM was 62.33 and 61.33%, when used Tris and citrate respectively, and without significant difference. In the Assaf the HOST values of thawed semen were 43.56 and 40.38%, while in Blackbelly were 40.19 and 38.16% respectively, both without significant differences. It is concluded that Tris showed better effect on individual motility but both of them had similar effect on sperm membrane integrity.
Start page
154
End page
160
Volume
21
Issue
2
Language
Spanish
OCDE Knowledge area
Ciencia veterinaria
Scopus EID
2-s2.0-85040342338
Source
Revista de Investigaciones Veterinarias del Peru
ISSN of the container
16823419
Sources of information: Directorio de Producción Científica Scopus