Introduction - The effect of fertilizer-use on fruit nutritional qualities of four plantain genotypes were determined. Materials and methods - Proximate composition, minerals, heavy metals and vitamin contents of two cultivars ('Agbagba' and 'Mbi-Egome') and two bred-hybrids ('PITA 14' and 'PITA 24') were evaluated across three fertilizer treatments - organic (20 t ha-1 year1 of poultry manure), inorganic (400 kg N + 600 kg K2O + 100 kg P2O5 ha-1 year1), and the combined half doses of organic + inorganic on split plot arrangement in randomized complete block design. Results - Analysis of variance showed that most of their proximate quality was not influenced by genotype or fertilizer treatment. However, ash, fibre, Mg and Ca contents were significantly (P<0.05) higher and similar in the organic and combined (inorganic + organic) plots. The hybrids had the highest mineral content particularly in the organic plot, while 'Mbi-Egome' had the lowest mineral content. Heavy metals (Pb and Cd) were present in negligible amounts across the treatments. All the vitamins (A, B1, B2, B3, B6 and C) were highest in fruits from the combined plot. The four genotypes were similar in vitamin content except for vitamins A and B6. 'PITA 24' had the highest vitamin A, whereas 'PITA 14' was noted for high vitamin B6. Conclusion - For optimum nutrients derivable in plantain fruits, organic or the combined application of organic and mineral fertilizers is recommended. The hybrids are recommended for adoption since they possess similar nutritional qualities with the cultivars.