The objective of this study was to develop and to analyze psychometric properties of two measurement scales that evaluate knowledge management practices and teamwork. An instrumental study was designed in a non-probabilistic sample of 334 research groups at Colombian higher education institutions. Measurement scales with 12 items for knowledge management practices and 18 items for teamwork were applied. The results confirm satisfactory adjustment of knowledge management practices to a two-factor structure and of teamwork to a three-factor structure. Validity evidence was provided based on test content and on relationships to other variables. The scores showed adequate levels of reliability. It is concluded that research groups scores on the two scales developed here have adequate psychometric properties and that, therefore, higher education institutions can use these scales.