Milk production requires large amounts of water and land, increasing water scarcity threatens the sustainability of livestock production. The objective of the study was to calculate the water footprint for the production of a liter of milk in livestock production systems located in the district of Florida, Amazonas. For this, 30 dairy farms were selected based on the farm area, grazing system, number of cows in production and milk production, obtaining three strata of 10 farms each. In each stratum, the direct consumption of water was determined as the water footprint of drinking, the indirect consumption of water as the water footprint of food, the use of water expressed as the water footprint of services from the description and quantification of the different activities that they use. water in the milk production phase and the water footprint to produce a liter of milk. For data processing, the software R v. 4.0.4. The results show that the water footprint for the production of a liter of milk is 1823.38 liters of water in stratum I (extensive), 736.80 liters in stratum II (stake) and 937.61 liters in stratum III (electric or controlled fence). The study contributed to the production of data that are still insufficient about the use and consumption of water in the milk production system, it also highlights the need to establish alternatives for the use and reuse of water resources.