Temporal and spatial fluctuations in food abundance may affect the feeding habits of vertebrates in tropical dry forests. We explored the effects of season and forest degradation in dietary patterns of the painted spiny pocket mouse (Lyomis pictus (Thomas, 1893)) (Heteromyidae) in a Mexican tropical dry forest. We used carbon (13C, 12C) and nitrogen (15N, 14N) stable isotope analyses to test the hypotheses that (i) L. pictus would increase its use of arthropods during the rainy season when seeds are less available on the forest floor and (ii) that L. pictus would increase its use of arthropods in degraded forest compared with conserved forest. Our hypotheses were wrong because assimilated biomass was derived almost exclusively from seeds in both seasons and the importance of arthropods was marginal in both sites. Examination of food remains in feces and cheek pouches confirmed these trophic patterns. Seed hoarding during the season of high seed availability probably allows L. pictus to subsist on a seed-based diet throughout the year in conserved and disturbed forests. This behavioral trait would enable L. pictus to maintain its specialized feeding habit in environments threatened by habitat degradation.