Title
Measuring the 3J HNHa coupling by a simple 2D-intra-HNCA IP/AP-E.COSY with simultaneous encoding of 15N chemical shift and 1J HaCa evolution
Date Issued
01 February 2022
Access level
metadata only access
Resource Type
journal article
Publisher(s)
Academic Press Inc.
Abstract
The 3J coupling values are commonly used in biomolecular NMR to extract structural information. Here we present a novel intra HNCA IP/AP E.COSY pulse sequence that allows to measure 3J HNHa coupling constants by a simple and rapid two-dimensional 1H-15N correlation experiment where the 15N frequency is encoded at the same time as the 1J HaCa coupling evolution. The advantage with respect to the conventional 3D HNCA E.COSY pulse sequence is the dimensionality reduction to a simple 2D experiment, which decreases acquisition time and facilitates data analysis. The performance of this new experiment is demonstrated with an ubiquitin sample at 500 MHz.
Volume
335
Language
English
OCDE Knowledge area
Química inorgánica, Química nuclear Bioquímica, Biología molecular
Scopus EID
2-s2.0-85121662582
PubMed ID
Source
Journal of Magnetic Resonance
ISSN of the container
10907807
Sponsor(s)
This study was funded by ICGEB early career grant (CRP/PER16-05-EC) and the Consejo Nacional de Ciencia, Tecnologia e Innovacion Tecnologica (CONCYTEC) - Programa Atracción de Investigadores Cienciactiva - Grant 008-2017-FONDECYT.
Sources of information: Directorio de Producción Científica Scopus