A serologic study was conducted to determine the prevalence of Parainfluenza-3 virus (PI3V), Syncytial Respiratoryvirus (RSV) and BovineViral Diarrhea virus (BVDV) in ruminants raised under harsh environmental conditions and poor management in the highlands of Cusco, Peru. Blood samples were collected in cattle (n = 66), alpaca (n = 21), and sheep (n = 152) for antibody detection against PI3V, RSV, and BVDV by serum neutralization test. The prevalence of PI3V, RSV, and BVDV was 81.8, 87.8, and 90.9% in cattle, 50.0, 49.3, and 28.3% in sheep, and 23.8, 4.7, and 15.8% in alpaca respectively. The antibodytiters ranged from 2 to >256 and the highest titers were found in bovine samples. The results indicate that these respiratoryviruses are widely distributed in the animals of this rural community and may play a key role in respiratory problems.