Introduction: The emergence of multi-resistant enterobacteria and extended spectrum betalactamase producers in external consultation patients with urinary tract infections represents a public health problem in Peru. Objectives. Molecularly characterize multi-resistant enterobacteria isolated from patients diagnosed with urinary tract infection from two departments of the Peruvian jungle. Materials and methods. A descriptive, observational and retrospective study o 61 uroculture isolates from the Peruvian jungle was conducted during 2017 -2018. Resistance profiles were identified using the MicroScan automated system®. A conventional polymerase chain reaction was used for the detection of the genes blacTx-M, blaTEM, blasHv. Results. The most common positive ESBL enterobacteria for each department were Escherichia coli, Madre de Dios 25% (10/40) and Ucayali 76.2% (16/21). For both departments the gene blacrx-M was the most prevalent with 41 % (25/61), followed by blaTEM with 24.6% (15/61) and blasHv with 16.4% (10/61). Antimicrobial susceptibility profile detected resistance levels with 72.6% for ampicillin, 82.3% cephalotin and 88.7% for nitrofurantoin. Conclusions. BLEE-producing multi-resistant enterobacteria strains in both departments were 57.4%; with the blacrx-M gene being the most common.