A total of 603 males belonging to 33 lineages were genotyped for 6 micorsatellites, one SNP and one INDEL to clarify the paternal genetic diversity of the Lidia cattle breed. The number of haplotypes identifying were 10 with frequencies ranging between 0.2% to 74%. All the haplotypes belonging to the haplogropus previously defined in European domestic bovine breeds. In 25 of the 33 lineaes only one haplotype was identifying with low values of haplotypic diversity in all the lineages. The majority of the genetic diversity was explained by genetic differences among lineages, as shown the AMOVA analysis (FST= 82%). The Network analysis shown two cluster clearly separated made up of those haplotypes belonging to each haplogroup.