Title
UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
Date Issued
17 December 2021
Access level
open access
Resource Type
journal article
Author(s)
Peñaranda K.
Mendoza-Rojas G.
Nakamoto J.A.
Dueñas E.
Alvarez D.
Publisher(s)
Cell Press
Abstract
Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.
Volume
2
Issue
4
Language
English
OCDE Knowledge area
Bioquímica, Biología molecular
Subjects
Scopus EID
2-s2.0-85118485880
PubMed ID
Source
STAR Protocols
ISSN of the container
26661667
Source funding
Validación clínica de la plataforma UnCovid para la detección molecular de SARS-CoV-2
Sponsor(s)
Funding text 1
We are very thankful to Dr. Marcos Milla for donating equipment that was used in this study and others. We would also like to thank all laboratory members of the Adaui and Milón groups for their help, support, and great working atmosphere. This work was supported by grants from the Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica [ 070-2020-FONDECYT ] to V.A. and [ 036-2019-FONDECYT-BM-INC.INV ] to P.M.
Funding text 2
We are very thankful to Dr. Marcos Milla for donating equipment that was used in this study and others. We would also like to thank all laboratory members of the Adaui and Mil?n groups for their help, support, and great working atmosphere. This work was supported by grants from the Fondo Nacional de Desarrollo Cient?fico, Tecnol?gico y de Innovaci?n Tecnol?gica [070-2020-FONDECYT] to V.A. and [036-2019-FONDECYT-BM-INC.INV] to P.M. Conceptualization and methodology, R.A. K.P. V.A. and P.M.; investigation, R.A. K.P. J.A.N. G.M.R. E.D. and D.A.; formal analysis, R.A. K.P. J.A.N. and P.M. writing ? original draft, supervision, and visualization, R.A. K.P. and P.M. writing ? review & editing, R.A. K.P. J.A.N. G.M.R. E.D. D.A. V.A. and P.M.; project administration, P.M. The authors declare no competing interests.
Sources of information:
Directorio de Producción Científica
Scopus