Objectives: To report the frequency and microbiological characteristics of the most commonly isolated corneal pathogens from infectious keratitis in a reference center in Mexico City. To describe the in vitro resistance and sensitivity patterns to the common use antibiotics in the commu- nity. Material and methods: Retrospective study, samples were obtained from corneas with diagnosis of infectious keratitis in the period January-December 2012. Results of cultures, stains and antibiograms were analyzed. Results: A total of 120 corneal scrapes were taken during the study period. Pathogens were obtained in 55 samples (45.83%), 49 (88.4%) of them attributed to bacterial organisms. Staphylococcus epidermidis was the most common isolated organism and Pseudomonas aeruginosa the most common Gram negative. We registered 5 cases of keratitis associated to Kocuria sp., all of them were 100% sensitive to moxifloxacin, ciprofloxacin, gentamicin and oxacillin. 89.58% of the isolates were sensitive to moxifloxacin and vancomycin, observing a general sensitivity of 85.71% to gentamicin. Resistance to ciprofloxacin was observed in 24.48% of the isolates and in 36.84% of the Pseudomonas isolates. All the Enterococcus faecalis isolates were moxifloxacin and ciprofloxacin resistant. Conclusions: The most common causative microorganisms were sensitive to quinolones; hence we recommend its use as empiric treatment in infectious keratitis. Resistance to oxacillin was observed in 39.35% of Gram positives, antibiotic multiresistance of the analyzed Enterococcus isolates was remarkable and five cases of Kocuria sp. keratitis, an opportunistic organism not usually associated to keratitis, were observed. © 2013 Sociedad Mexicana de Oftalmología.