Title
Real-Time Dynamics of Ribosome-Ligand Interaction by Time-Resolved Chemical Probing Methods
Date Issued
01 January 2007
Access level
metadata only access
Resource Type
book part
Author(s)
University of Camerino
Publisher(s)
Academic Press Inc.
Abstract
Three protocols to perform time-resolved in situ probing of rRNA are described. The three methods (chemical modification with DMS and rRNA backbone cleavage by hydroxyl radicals generated by either K-peroxonitrite or Fe(II)-EDTA) make use of a quench-flow apparatus and exploit reactions that are faster than the interactions of ribosomal subunits with their ligands. These methods allow the investigation of the path and dynamics, in a ≅ 50 to 1500 ms time range, of the binding and dissociation of ribosomal ligands. © 2007 Elsevier Inc. All rights reserved.
Start page
45
End page
58
Volume
430
Language
English
OCDE Knowledge area
Bioquímica, Biología molecular
Scopus EID
2-s2.0-38449101681
PubMed ID
Source
Methods in Enzymology
Resource of which it is part
Methods in Enzymology
ISSN of the container
00766879
ISBN of the container
978-012373969-8
Sponsor(s)
The financial support of PRIN 2005 grants from the Italian MIUR to COG and CLP is gratefully acknowledged.
Sources of information:
Directorio de Producción Científica
Scopus