cris.boxmetadata.label.title
Real-Time Dynamics of Ribosome-Ligand Interaction by Time-Resolved Chemical Probing Methods
cris.boxmetadata.label.dateissued
01 browse.startsWith.months.january 2007
cris.boxmetadata.label.accesslevel
metadata only access
cris.boxmetadata.label.resourcetype
book part
cris.boxmetadata.label.authors
Fabbretti A.
MILON MAYER, POHL LUIS
Giuliodori A.
Gualerzi C.
Pon C.
University of Camerino
cris.boxmetadata.label.publisher
Academic Press Inc.
cris.boxmetadata.label.abstract
Three protocols to perform time-resolved in situ probing of rRNA are described. The three methods (chemical modification with DMS and rRNA backbone cleavage by hydroxyl radicals generated by either K-peroxonitrite or Fe(II)-EDTA) make use of a quench-flow apparatus and exploit reactions that are faster than the interactions of ribosomal subunits with their ligands. These methods allow the investigation of the path and dynamics, in a ≅ 50 to 1500 ms time range, of the binding and dissociation of ribosomal ligands. © 2007 Elsevier Inc. All rights reserved.
cris.boxmetadata.label.citationstartpage
45
cris.boxmetadata.label.citationendpage
58
cris.boxmetadata.label.volume
430
cris.boxmetadata.label.language
English
cris.boxmetadata.label.ocdeknowledgeArea
Bioquímica, Biología molecular
cris.boxmetadata.label.doi
cris.boxmetadata.label.scopusidentifier
2-s2.0-38449101681
cris.boxmetadata.label.pubmedidentifier
cris.boxmetadata.label.source
Methods in Enzymology
cris.boxmetadata.label.partofresource
Methods in Enzymology
cris.boxmetadata.label.containerissn
00766879
cris.boxmetadata.label.containerisbn
978-012373969-8
cris.boxmetadata.label.sponsor
The financial support of PRIN 2005 grants from the Italian MIUR to COG and CLP is gratefully acknowledged.
peru-layout.shadow-copies
Directorio de Producción Científica
Scopus