Title
CRISPR-Cas Activators for Engineering Gene Expression in Higher Eukaryotes
Date Issued
01 October 2020
Access level
open access
Resource Type
review
Author(s)
Universidade de São Paulo
Publisher(s)
Mary Ann Liebert Inc.
Abstract
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.
Start page
350
End page
364
Volume
3
Issue
5
Language
English
OCDE Knowledge area
BiologÃa celular, MicrobiologÃa
BioquÃmica, BiologÃa molecular
Scopus EID
2-s2.0-85095452575
PubMed ID
Source
CRISPR Journal
ISSN of the container
25731599
DOI of the container
10.1089/crispr.2020.0064
Sponsor(s)
J.A.C.M. and M.J.S. are part of a team supported by the Advanced Plant Technologies program, DARPA Award HR001118C0146. M.H.Z. is supported by an NIH NIGMS Biotechnology Training grant NIHT32GM008347.
Sources of information:
Directorio de Producción CientÃfica
Scopus