Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge. In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes. Pretreatment resulted in compositional modifications in the material, mainly regarding the cellulose and lignin contents. The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32–35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively. In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38–46 g and 32–38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step. HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.