The present study aimed to investigate the reliability and construct validity of a new version of the Brief Job Stress Questionnaire (New BJSQ), which measures an extended set of psychosocial factors at work by adding new scales/items to the current version of the BJSQ. Additional scales/items were extensively collected from theoretical models of job stress and similar questionnaires in several countries. Scales/items were field-tested and refined through a pilot Internet survey. Finally, an 84-item standard version questionnaire, a 63-item recommended set, and a 23-item short version (141, 120, and 80 items in total when combined with the current 57-item BJSQ) were developed. A nationally representative survey was administered to employees in Japan (n = 1633) in 2010/2011 to examine the reliability and construct validity. As a result, most scales showed acceptable levels of internal consistency (Cronbach’s alpha) and test-retest reliability over one year. Principal component analyses showed that the first factor explained 50 % or greater proportion of the variance in most scales. A scale factor analysis and a correlation analysis showed that these scales fit the proposed theoretical framework. These findings provided a piece of evidence that the New BJSQ scales are reliable and valid. The New BJSQ can be a useful instrument to evaluate psychosocial work environment and positive mental health outcomes in the workplace.