Title
Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings
Other title
Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas
Date Issued
01 January 2019
Access level
open access
Resource Type
journal article
Author(s)
Publisher(s)
Universidad Nacional Mayor de San Marcos
Abstract
The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression.
Start page
1129
End page
1142
Volume
30
Issue
3
Language
Spanish
OCDE Knowledge area
Biotecnología agrícola, Biotecnología alimentaria
Biotecnología ambiental
Acuicultura
Subjects
Scopus EID
2-s2.0-85073190489
Source
Revista de Investigaciones Veterinarias del Peru
ISSN of the container
16823419
Source funding
Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica
Sponsor(s)
32-2015-FONDECYT
Sources of information:
Directorio de Producción Científica
Scopus