Title
Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru
Date Issued
01 January 2015
Access level
open access
Resource Type
journal article
Author(s)
Duarte C.G.
Bonilla C.
Guimarães G.
Machado De Avila R.A.
Mendes T.M.
Silva W.
Universidade Federal de Minas Gerais
Publisher(s)
Elsevier Ltd
Abstract
In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
Start page
37
End page
40
Volume
93
Language
English
OCDE Knowledge area
Toxicología
Subjects
Scopus EID
2-s2.0-84919935913
PubMed ID
Source
Toxicon
ISSN of the container
00410101
Sponsor(s)
This research was supported by Instituto Nacional de Salud (INS), Lima, Peru (R.D. N°1099-2009-DG-OGITT-OPE/INS) and executed by specific agreement with the Universidad Nacional Mayor de San Marcos, Lima, Peru. The authors gratefully acknowledge the financial support of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil – CAPES (TOXINOLOGIA N o 23038000825/2011-63 ), Fundação de Amparo a Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG) and by funds of the INCTTOX PROGRAM of Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq) .
Instituto Nacional de Ciência e Tecnologia em Toxinas - INCTTOX
Sources of information:
Directorio de Producción Científica
Scopus