This article presents an algorithm for obtaining the weight and concentration of DNA samples from agarose gel images using digital image processing techniques. The purpose of this algorithm is to obtain much more accurate results than the results obtained by experts using their visual sense. The algorithm is based on capturing the DNA samples and comparing them with DNA molecular weight markers using digital image processing techniques to find the concentration, which is the intensity of the samples, and the weight of the samples. The proposed algorithm achieved a moderate “level of agreement” in the results, as calculated by kappa, compared with the expert results of a total of six images, containing 51 DNA samples in total. These DNA samples open source is from flies.