Ciprofloxacin is one of the most widely used antibiotics and knowledge of concentration in the urine is of great significance for pharmacokinetic and environmental studies. In this work, the Poly (Acrylic Acid – Ethylene Glycol Dimethacrylate) Molecularly Imprinted Polymer was optimized as a adsorbent in solid phase extraction (MISPE) for Ciprofloxacin. It is desirable because they are adsorbent materials highly selective to their template molecule. The MIP resulted in maximum sorption capacities of 282 μmol g-1 in batch studies and in μmol g-1 in the breakthrough curve studies. MISPE was applied in synthetic urine to test its viability. The limit of detection was 70 ng mL-1. The enrichment factor of the method was 5.32, and recovery in urine ranged from 94.2% to 120.8%. These results showed the applicability of this optimized MISPE.