The aim of this study was to evaluate the serological frequency of canine herpesvirus type 1 (CHV-1) in dogs with a history of reproductive problems (infertility, embryo reabsorption, abortions and stillbirths) in kennels in Lima, Peru. Forty serum samples were obtained in 2019 from dogs from nine commercial kennels. The presence of anti-CHV -1 antibodies was determined using a commercial indirect ELISA kit. Twenty-six samples were positive (65%), with 37.5% (9/24) of positive females being in estrus at the time of sampling. A high exposure to CHV-1 is reported in kennels in Lima together with a seropositivity of eight times the cut-off point.