Objectives: To report the frequency and microbiological characteristics of the most commonly isolated corneal pathogens from infectious keratitis in a reference center in Mexico City. To describe the in vitro resistance and sensitivity patterns to the common use antibiotics in the community. Material and methods: Retrospective study, samples were obtained from corneas with diagnosis of infectious keratitis in the period January- December 2011. Results of cultures, stains and antibiograms were analyzed.Results: A total of 154 corneal scrapes were taken during the study period. Pathogens were obtained in 68 samples (44.15%), 60 (88.2%) of them attributed to bacterial organisms. Staphylococcus epidermidis (S. epidermidis) was the most common isolated organism and Pseudomonas aeruginosa the most common Gram negative. The 90.57%, 83.02% and 78.85% of the isolates were sensitive to ciprofloxacin, vancomycin and gentamicin respectively. Resistance to ceftazidime was observed in 86.27% of the isolates and in 100% of the Pseudomonas isolates. All the S. epidermidis isolates were oxacillin and tobramycin resistant. Conclusions: The most common causative microorganisms were sensitive to quinolones; hence we recommend its use as empiric treatment in infectious keratitis. An absolute resistance to tobramycin and oxacilina was observed in Gram positives as well a generalized resistance to cephalosporins. ©2012 Sociedad Mexicana de Oftalmología.