Title
A protocol to differentiate nociceptors, mechanoreceptors, and proprioceptors from human pluripotent stem cells
Date Issued
17 June 2022
Access level
open access
Resource Type
journal article
Author(s)
Zeltner N.
Universidad de Georgia
Publisher(s)
Cell Press
Elsevier B.V.
Abstract
Human pluripotent stem cells (hPSCs) show promise for studying diseases affecting cell populations that are not easily available, including sensory neurons (SNs). Here, we present a differentiation protocol in chemically defined conditions to generate peripheral SNs from hPSCs. We describe four main steps: expansion of hPSCs, neural crest cell (NCC) differentiation, NCC dissociation and replating, and sensory neuron (SN) differentiation. This protocol enables generation of a mechanoreceptor-enriched culture or a population containing all three SN subtypes (nociceptors, mechanoreceptors, and proprioceptors). For complete details on the use and execution of this protocol, please refer to Saito-Diaz et al. (2021).
Volume
3
Issue
2
Language
English
OCDE Knowledge area
Biología celular, Microbiología
Bioquímica, Biología molecular
Subjects
Scopus EID
2-s2.0-85126695978
PubMed ID
Source
STAR Protocols
ISSN of the container
2666-1667
Sponsor(s)
We want to thank Dr. Lorenz Studer for his support at the initial stages of this work and Jessica McAlpine for critical reading of the manuscript. This work was funded by faculty start-up funds from the University of Georgia to N.Z. and NIH/NINDS 1R01NS114567-01A1 to N.Z. Schematics were done using Biorender.com. N.Z. and K.S-D. conceived and designed the experiments; K.S-D. conducted the experiments; K.S-D. and N.Z. analyzed and interpreted the data; K.S-D. and N.Z. wrote the manuscript; N.Z. provided mentoring, financial, and administrative support and approved the final version of manuscript. The authors declare no competing interests. This work is linked to the patent ‘Compositions and methods for making sensory neurons’, Serial number is 63/127,9, held by the University of Georgia.
We want to thank Dr. Lorenz Studer for his support at the initial stages of this work and Jessica McAlpine for critical reading of the manuscript. This work was funded by faculty start-up funds from the University of Georgia to N.Z. and NIH/ NINDS 1R01NS114567-01A1 to N.Z. Schematics were done using Biorender.com .
Sources of information:
Directorio de Producción Científica
Scopus