Milk quality is affected by the concentration of lead (Pb) in soil and pasture grasses used to raise cattle, especially in areas near mining-metallurgical complexes. In this study, the Pb content of soil and its transfer to grass and dairy milk in an area located to 20 km from the La Oroya Metallurgical Complex in Peru (altitude, >3,700 m s.a.l) was measured. Twenty soil samples (0-30 cm depth), 20 grass samples were collected, and 0.5 L of milk was obtained from 20 lactating cow in the communal cowshed. The Pb concentration (mg/kg) was quantified by flame atomic absorption spectrometry using a commercial Pb standard as quality control. The Pb average concentration in the soil, grass, and milk samples were 217.81 ± 39.48, 20.09 ± 2.83, and 0.58 ± 0.018 mg/kg (P < 0.01), respectively. The transfer factor (ratio of metal concentration) for Pb from soil to grass and from grass to milk was 0.095 and 0.031, respectively. The soil, grass, and milk samples all presented high Pb concentrations, with the milk samples containing 29-fold more Pb than the safety limit established by European regulations and were not suitable for human consumption or the manufacture of cheese, yogurt, and other derivatives. Our findings demonstrate that action to remediate these soils is critically needed.