The paper Bacterial succession in bioheap leaching (Brierley, 2001) showed the search for methods to analyze the microbial dynamics in bioleaching industrial processes as a key challenge for advancing commercial bioheap applications. "Chemical and physical conditions within bioheaps change radically from the time the bioheap is stacked and inoculated until bioleaching is completed." The results from a comprehensive monitoring program by culturing and molecular techniques in an industrial bioleaching process for run-of-mine (ROM) low grade copper sulfide ore in Chile is summarized. The analysis of the compiled information permits an understanding of changes in microbial substrates availability, chemical and physical conditions. A data mining technique, called decision trees was used to analyze the information and to establish rules that represent patterns in the data. The bacterial succession in bioheap leaching solutions allowed the leaching cycle stages to be described as: i) Acid conditioning and soluble copper releasing, ii) Chalcocite Bacterial leaching (ferrous oxidation); iii) Chalcocite Bacterial leaching (ferrous and reduced sulfur compounds - RISC oxidation); iv) Bacterial leaching of sulfide minerals with higher rest potentials (pyrite and covellite), and v) Bacterial oxidation of remnant sulfide minerals and RISC. The impact of other factors on microbiology, such as the solvent-extraction process, aspects of the industrial design and operation are also discussed. © 2010 Elsevier B.V. All rights reserved.