Genetic resistance to potato cyst nematode is considered as one of the most effective means of increasing yield and reducing nematode infestation levels in potato fields. In this study, resistance to this nematode was successfully transferred from diploid tuber-bearing Solanums to the tetraploid gene pool using a 4x-2x breeding approach. More specifically, resistance from Solanum vernei, S. sparsipilum and haploids of S. tuberosum group Andigena was introgressed into conventional tetraploid clones, using first division restitution (FDR) 2n gametes. Furthermore, some of the FDR diploid parents had similar breeding values as advanced resistant tetraploid clones which were developed only after several cycles of selection against the potato cyst nematode.