Objective: The objective was to provide psychometric evidence of the 14-item and 10-item version of the Perceived Stress Scale in nursing professionals from Peru. Method: Data on 2848 professionals (92.27% women) between 23 and 69 years old (M = 21.87; SD = 10.76) was extracted from ENSUSALUD 2015. An exploratory structural equation modelling (ESEM) was applied with Mplus 7.0 software to analyse several measurement models in the PSS: unidimensional, bidimensional, models with method factor, and bifactor. Finally, the reliability was analysed. Results: The two-factor structure obtained adequate fit indices, and acceptable factorial loadings (>. 50), while the unidimensional model has poor statistical support. The construct and score reliability was also adequate. Conclusion: The two-factor model of the PSS-10 and PSS-14 presents adequate psychometric properties to expand its use to empirical research.