We describe two new species of Bryophryne from the Region of Cusco, Provincia de La Convencin in southern Peru, increasing the number of currently known Bryophryne to eight. One of the new species is the second known species of Bryophryne that has a tympanic annulus and tympanic membrane. Males of this species have vocal slits, a vocal sac, and call from inside moss. It is readily distinguished from all its congeners by having a blackish-brown venter with yellow, orange, or pale pink blotches. This species is found at elevations of 38003850 m in the puna along the road from Vilcabamba to Pampascona. The second new species has the venter orange and pale gray mottled in males, whereas the venter is black and pale gray mottled in the single female. It was found inside bunches of the Peruvian feather grass (Stipa ichu) in the puna of Abra Mlaga next to the road at an elevation of 4000 m. A map showing the type localities of all currently known species of Bryophryne is presented. © 2010 by The Herpetologists' League, Inc.