Grape waste causes great commercial and environmental losses due to economic losses and bad odors that they emit when decomposing, due to this this work gives an innovative way to generate electricity for the benefit of society. Microbial fuel cells were used as a technology for the generation of electricity, which were manufactured at low cost using graphite and zinc electrodes; while a 500 mL bottle was used as a substrate collection chamber (grape residues). It was possible to generate maximum values of 0.87 ± 0.064 V and 2.1 ± 0.45 mA, while the pH values increased from the first 3.6 to 5.25 ± 0.32. While the Brix degrees slowly decreased from the first day. The value of the maximum power density was 3.08 ± 0.0345 W/cm2 at a current density of 274.17 mA/cm2 at a maximum voltage of 0.81 ± 0.13 V. Finally, the micrographs of the anode electrode show the modification of a smooth surface (in its initial state) to a rough surface (the last day of monitoring). This research gives a new way of using grape waste for the generation of bioelectricity.