In this project it is expected to obtain the optimal conditions of the microalgae culture processes by modifying the parameters of pH, temperature, lighting, nutrient concentration in order to increase the productivity of biomass in the crop and reduce operating costs in the extraction of the Biodiesel generation process. using domestic wastewater as a culture medium. The results obtained serve to increase the efficiency of the microalgae culture process in photobioreactor (50 mL, 250 mL and 1000 mL) through the use of industrial wastewater containing nutrients and good control of the operating parameters. Microalgae was obtained in domestic wastewater, after three weeks, in a volume of 1L: 0.16 g of dried clorella sp microalgae. With a 1600% growth. The wastewater lowered its phosphorus concentration from 5.3 ppm to 3.8 ppm.