Title
The DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli
Date Issued
01 March 2006
Access level
metadata only access
Resource Type
journal article
Author(s)
Ayala J.A.
Centro de Biología Molecular Severo Ochoa
Abstract
The gene (pbp4B) encoding a putative DD-carboxypeptidase has been deleted in Escherichia coli and it is shown to be not essential for cell division. Disruption of the gene in a genetic background where all putative activities of DD-carboxypeptidases and/or DD-endopeptidases had been eliminated indicates that these activities are not required for cell growth in enterobacteria. The penicillin-binding capacity and a low DD-carboxypeptidase activity of PBP4B are demonstrated. © Springer-Verlag 2006.
Start page
23
End page
27
Volume
185
Issue
1
Language
English
OCDE Knowledge area
Bioquímica, Biología molecular
Biología celular, Microbiología
Scopus EID
2-s2.0-33644532221
PubMed ID
Source
Archives of Microbiology
ISSN of the container
03028933
Sponsor(s)
Acknowledgement We thank Dominique Mengin-Lecreulx for help with Datsenko technique, and Kevin D. Young for kindly providing us with mutant strain CS802-2. This work was supported by grant number BIO2000-0235 from the Spanish Ministry of Science and Technology and grant number LSHM-CT-2003-503335 (European Union). The institutional help of the Foundation Ramon Areces to the CBMSO is acknowledged.
Sources of information:
Directorio de Producción Científica
Scopus