Herein, based on molecular and morphological evidence, we report the first record of Eumops bonariensis (Peters 1874) and Pteronotus fuscus (Allen 1911) for Peru, representing a range extension of at least 1580 km (to Ucayali department) and 1860 km (to San Martín department) from the nearest records, respectively; and confirm that Pteronotus rubiginosus (Wagner 1843) is absent in Peru. We also provide comments on the taxonomy, distribution, and natural history of these species. This study highlights the importance of exploring unsampled localities and doing detailed morphological studies and extensive use of molecular methods.