Title
Determination of sphingomyelinase-D activity of Loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidase
Date Issued
15 March 2011
Access level
open access
Resource Type
journal article
Author(s)
Gomes M.
Guimarães G.
Frézard F.
Kalapothakis E.
Minozzo J.
Chaim O.
Veiga S.
Oliveira S.
Universidade Federal de Minas Gerais
Abstract
Based on degradation of sphingomyelin/cholesterol liposomes containing entrapped horseradish peroxidase, we evaluated the Sphingomyelinase-D (SMase-D) activity of scorpion, spider and snake venoms by monitoring spectrophotometrically the product of oxidation of HRP released. The results indicate that Loxosceles crude venoms (Loxosceles intermedia, Loxosceles laeta, Loxosceles gaucho and Loxosceles similis) displayed SMase-D activity in a concentration-dependent manner. Furthermore, this activity was blocked by the anti-loxoscelic antivenom. However, Tityus serrulatus scorpion venom, Phoneutria nigriventer spider venom and Bothrops jararaca, Crotalus durissus, Lachesis muta and Micrurus frontalis snake venoms did not show measurable SMase-D activity. © 2011 Elsevier Ltd.
Start page
574
End page
579
Volume
57
Issue
4
Language
English
OCDE Knowledge area
Toxicología
Subjects
Scopus EID
2-s2.0-79952362975
PubMed ID
Source
Toxicon
ISSN of the container
00410101
Sponsor(s)
Funding text
We would like to express gratitude to Dr. Michael Richardson for his critical review of this manuscript. This research was supported by Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), the INCTTOX PROGRAM of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Sources of information:
Directorio de Producción Científica
Scopus