Title
Neutralizing capacity of antibodies elicited by a non-toxic protein purified from the venom of the scorpion Tityus serrulatus
Date Issued
01 February 1997
Access level
metadata only access
Resource Type
journal article
Author(s)
Fundação Ezequiel Dias
Abstract
Polyclonal rabbit antibodies raised against a non-toxic protein (TsNTxP) purified from the toxic fraction of the crude venom of Tityus serrulatus can neutralize the effects of the venom. The antigenic specificities of anti-TsNTxP were compared by an indirect enzyme-linked immunosorbent assay using TsNTxP, TstFG50 (toxic fraction of venom that represents most of the toxicity of the crude venom), and crude venoms from T. serrulatus, T. bahiensis, T. cambridgei, T. stigmurus, Androctonus australia Hector and Centruroides sculpturatus to coat microtitration plates. The anti-TsNTxP antibodies had a comparable high cross-reactivity with the toxic fraction and crude venom of T. serrulatus, moderate binding capacity for T. bahiensis, T. cambridgei, T. stigmurus and were unable to recognize the venoms of A. australis Hector and C. sculpturatus. Quantities of venom equivalent to 20 LD50 were effectively neutralized by 1 ml of the anti-TsNTxP serum. This result shows that this protein may be of interest in the production of antivenoms for clinical use.
Start page
213
End page
221
Volume
35
Issue
2
Language
English
OCDE Knowledge area
Toxicología
Scopus EID
2-s2.0-0031081491
PubMed ID
Source
Toxicon
ISSN of the container
00410101
Sponsor(s)
Funding text
Acknowledgements-This research was supported by grants from Conselho National de Desenvolvimento Cientifico e Tecnologico (CNPq), FundacQo de Amparo a Pesquisa de Minas Gerais (FAPEMIG) and Fundacao Ezequiel Dias (FUNED). The technical assistance of Dario Jose de Souza with the in ho assays is gratefully acknowledged.
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