The recently described bat Lasiurus arequipae Málaga, Díaz, Arias & Medina, 2020 is endemic to Peru. Lasiurus arequipae is currently known to occur at two localities in the department of Arequipa and one locality in the department of Tacna in southwestern Peru. Here, based on morphological and molecular data, we present a fourth locality that represents the southernmost record of this species and extends its known range 62 km south from the nearest locality in Tacna. Additionally, we provide information some aspects on the natural history and acoustic profile of L. arequipae.