The use of sodium chloride for the preservation of pelts and skins destined for tanning is a very widespread technique in Peru, therefore the quantities used of this salt represent high concentrations of chlorides in the liquid effluents that are generated, which can affect the environmental quality of ecosystems and human health. The present study aimed to estimate an allowable concentration of chlorides in tanning effluents using ecotoxicological tools so that it would serve as a guide for the tanning industry and the authorities in their efforts to establish better conservation practices and effluent control parameters, which are currently absent from national environmental legislation. The permissible concentration of chlorides was determined using bioassays with seeds of Lactuca sativa species (lettuce), which was exposed to soaking effluents obtained from local tanneries. The studied species was determined based on sensitivity tests to zinc sulfate (ZnSO4 ) applied to three commercial lettuce species. The selected lettuce seed presented a germination rate higher than 90%, a CV lower than 30% and an IC50 of 38.97 mg/L of Zn+2 with a confidence interval between 19.30 mg/L and 58.65 mg/L of Zn+2. The admissible guide concentration determined for chlorides in soaking effluents from IC50 was 582.30 mg/L with a minimum value of 258.45 mg/L and a maximum of 906.15 mg/L. Additionally, because of the presence of other pollutants in the soaking effluents, such as bactericides, organic matter, enzymes and salts added in soaking process, tests with synthetic solutions of sodium chloride at the same concentrations of the soaking effluents were conducted, to evaluate the effect produced by those substances.