The prevalence of the bovine herpesvirus 1 (BHV-1) was studied in cattle raised under an extensive rearing system and without historyof vaccination. Herds were located in the province of San Pablo, department of Cajamarca, Peru. Blood samples from 480 bovine were collected for detection of antibodies against BHV-1 by viral neutralization test. The seroprevalence was 0.6 ± 0.7% (3/480). The three positive Holstein cows, older than 6 years, were part of the same herd and the antibodytiters were 1:64 (2) and 1:32 (1). The results indicated that the BHV-1 is now widely distributed in cattle of the studied region.