The effect of two commercial enzymes (Allzyme Vegpro® and RovabioTM Excel) on the in situ digestibility and kinetics of the dry matter of sugarcane bud (Saccharum officinarum) was evaluated. The substrate was dried, grounded, passed through a 3 mm sieve, and incubated with the enzymes for 30 minutes. The concentration of the enzymes was 1% of the substrate. In addition, a substrate without treatment was considered as control treatment. A randomized experimental design, with four periods, three treatments and two replicates per period was used. The samples (1.8 g) in dracon bags were placed in the first stomach compartment of one fistulated alpaca and six digestion times (6, 12, 24, 48, 72 and 96 h) were applied. The parameters evaluated in digestion kinetics were initial digestibility, degradable fraction, degradation rate, potential digestion and average time. The dry matter (ISDMD) in situ digestibility did not improve; however statistical differences (p<0.05) were found at 6, 12 and 48 hours but without biological significance. There were no differences among treatments for the studied variables. It is concluded that the dose used of fibrolytic enzymes does not increase digestibility or kinetics of digestion of sugarcane bud.