Natural and synthetic endocrine disrupting chemicals (EDCs) are released into the environment, mainly through sewage treatment systems, before reaching the receiving bodies. It is crucial to maintain ongoing efforts to develop, validate and/or update the current approaches for the effective removal of EDCs. White-rot fungi (WRF) secrete mediators of high molecular weight, increasing the range of compounds potentially biodegradable. The enzymatic membrane reactor (EMR) configuration detailed in this chapter consists of a continuous stirred tank reactor coupled to an ultrafiltration polyethersulfone membrane with a nominal molecular weight cut-off of 10kDa, which allows the recovery and recycling of the enzyme to the reaction vessel. The most common transformation products (TPs) from advanced oxidation processes (AOPs) are obtained by the breakdown of the EDC structure, yielding molecules with lower molecular weight than that of the parent compound and reduced estrogenic activity.