Background. Emotion Regulation comprises a set of strategies (cognitive, emotional, and physiological) that allow individuals faced with internal or external stimuli to manage their emotional response, to adapt to the environment, and to achieve goals. The Emotion Regulation Questionnaire (ERQ) is used to assess Emotion Regulation. It has been translated into several languages (including Spanish) and has been adapted around the world, but its psychometric properties have not been tested in Ecuador. Objective. To confirm the bifactor structure of the Emotion Regulation Questionnaire and its reliability in a sample of Ecuadorian college students. Design. A quantitative and instrumental study using Confirmatory Factor Analysis with Robust Maximum Likelihood estimation. The sample consisted of 400 participants (62.5% women), aged 18 to 25 (M = 21.1; SD = 1.95) from two universities in Ecuador and seven different undergraduate courses.